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03-17-2012, 09:15 AM
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#1 |
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Junior Member
Location: uk Join Date: Mar 2012
Posts: 4
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I'm a beginner! Help?
Hi guys, I'm a beginner I'm trying to understand next gen sequencing. Basically I'm writing a project up in which I extracted DNA/RNA for next generation sequencing. I'm writing at why I would require high molecular weight DNA/RNA in order to construct libraries. I don't really understand what libraries this means? I think it could mean the sheared fragments that are clonally amplified before using NGS on them, but I'm very stuck. Are there any papers you could link me to which would make this sort of thing clearer?
Thanks alot! |
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03-18-2012, 05:19 PM
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#2 |
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Senior Member
Location: Bethesda MD Join Date: Oct 2009
Posts: 509
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Most protocols explain the rationale for using high MW material; that would be a good place to start.
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11-20-2012, 07:01 PM
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#3 |
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Junior Member
Location: malaysia Join Date: Jan 2012
Posts: 2
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What would the explanation be? I've not seen a direct reason the protocols suggesting it so far.
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11-20-2012, 07:25 PM
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#4 | |
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Senior Member
Location: Hong Kong Join Date: Dec 2008
Posts: 350
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Quote:
__________________
Marco |
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