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#1 | ||
Member
Location: Germany Join Date: Sep 2009
Posts: 21
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Dear TopHat users,
I have 51bp-long, paired-end reads. I'm using TopHat v1.0.13, with Bowtie 0.11.3, on a Linux computer with x86_64 architecture. I run TopHat with the following parameters: tophat -G /home/../Homo_sapiens.GRCh37.58VN.gff -r 80 --no-novel-juncs -a 8 -m 0 -I 1000000 -g 100 -o ~/tophat_sj --segment-mismatches 2 --segment-length 25 --max-segment-intron 1000000 /....../hg18 /......./s_1_1.fastq /...../s_1_2.fastq Here is an example from my GFF file: chr11 protein_coding gene 127926 139099 . - . ID=ENSG00000230724;Name=AC069287.3 Quote:
Here ist the result in the output 'junctions.bed' file. Quote:
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