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09-20-2016, 01:17 PM
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#1 |
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Junior Member
Location: london Join Date: Nov 2013
Posts: 5
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Quality per tile
Hi there,
I have three samples which were sequenced on a MiSeq. I have attached the R1 and R2 quality per tile for sample 3 and R2 for samples 1 and 2. For the reverses I get a quality per tile fail for R2 with almost identical plots for all three samples. After trimming (which normally works pretty well!!) things seem worse. Is there something else I should be doing (e.g. re-trimming/removing affected reads)? Any help/advice would be great!!! Thanks in advance!!! |
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| illimina miseq, miseq 250bp, quality per tile |
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