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Old 10-29-2010, 09:41 AM   #1
Junior Member
Location: UK

Join Date: Jul 2010
Posts: 3
Default RNAseq from single cell RT?

i am working with a very limited amount of laser captured cells. RNA purification is extremely problematic in this setting, but direct lysis/cDNA amplification works very well.
how should i proceed from this first "classical" RT step to NGS?
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library preparation, rnaseq, single cell

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