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Old 02-05-2010, 12:31 PM   #1
Junior Member
Location: Jerusalem Israel

Join Date: Feb 2010
Posts: 1
Default Poly A cDNA- 454 Titanium

My name is Amir. I just finished my first run on the Titanium 454 using polyA cDNA as atemplate. The output was quite low around 100Mb and contain large stretches of Ts.
I will be happy tp get responsese abput the possibility to run PolyA cDNA on the Titanium. The facilty I used claim that there is no problem running polyA cDNA on the Titanium but it seems that most places do not do it.
amirsherman is offline   Reply With Quote
Old 02-06-2010, 08:30 AM   #2
Location: Oslo, Norway

Join Date: Nov 2008
Posts: 415

Stretches of T in cDNA reads cause problems during sequencing, as they result in a large flash of light. Neighboring wells are influenced by this and might fail, causing a reduced run output.

Best to avoid the polyA tail next time...
And, perhaps switch sequencing provider.
flxlex is offline   Reply With Quote

454 poly a low yield

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