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  • sff_extract paired end extraction error

    I have been trying to run sff_extract using the -l flag to
    extract the paired end read from my sff file but I receive
    the following error.

    brian@Gaff[Pairedend_extract] sff_extract -o testrun -l
    /home/brian/Neo_home/test/Pairedend_extract/LINKER/Linker.fasta
    /home/brian/Neo_home/test/FE4758201.sff
    [ 1:21PM]
    Working on '/home/brian/Neo_home/test/FE4758201.sff':
    Creating temporary sequences from reads in
    '/home/brian/Neo_home/test/FE4758201.sff' ... done.
    Searching linker sequences with SSAHA2 (this may take a
    while) ... Input parameter error for "-solexa"


    An error occured during the SSAHA2 execution, aborting.

    I am unsure what exactly is causing the error as SSAHA2
    works fine outside of sff_extract and sff_extract works perfectly
    when not using the -l flag

    regards

    Brian

  • #2
    Wrong version of ssaha, upgrade to one that knows the "-solexa" flag :-)

    B.

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