Hello, there,,
Recently, I perfomed the GS FLX Sequencing run using 70X75 PTP, 4 Regions Gasket.
Unfortunately, I got some weired results.
Actual keypass reads# of my library was about ~170000, but most of them(~70%) were filtered out due to short read length. Control reads were also similar results.
This is the first time that I get this kind of problem while I'm using 454's Genome sequencer.
I'm supspecting of reagent itself or instrument fluidic part.
Is there anyone who can hlep me or suffering from this kind of issue?
Recently, I perfomed the GS FLX Sequencing run using 70X75 PTP, 4 Regions Gasket.
Unfortunately, I got some weired results.
Actual keypass reads# of my library was about ~170000, but most of them(~70%) were filtered out due to short read length. Control reads were also similar results.
This is the first time that I get this kind of problem while I'm using 454's Genome sequencer.
I'm supspecting of reagent itself or instrument fluidic part.
Is there anyone who can hlep me or suffering from this kind of issue?
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