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Old 12-21-2014, 06:38 PM   #1
weigrc
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Default An extra RNA peak positioned between 16s and 23s RNAs, what it could be??

Hello everyone,

As captioned, 2 (#01 and #03) and 2 (#06 and #09) RNAs (refer to attached) were harvested from the same E.coli in growth phase and post-growth phase, respectively.

A strange RNA peak occurs at between 16s and 23s rRNAs in Sample #06 and #09.

These RNA samples are planing to do NGS RNA-Seq with ribo-zero process.


Thanks and happy holidays,
Wei
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File Type: pdf Extra RNA peak between 16s and 23s.pdf (313.0 KB, 33 views)
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Old 12-22-2014, 12:47 PM   #2
cliffbeall
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It's probably a cleavage product of the 23S, which is why those two samples have RIN of 9.4 while the other two have RIN 10.
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Old 12-22-2014, 04:38 PM   #3
weigrc
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Dear Cliffbeall,

Thanks for the idea, yes, it can be such case. And anyone has a similar phenomena can share with? I am a bit worry about if the extra peak is a contamination that will affect the RNA sequencing result a lot.

Merry Christmas!!
Wei
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