I cannot confirm or deny whether random hexamers are used in the PCR step during the process. My guess is that there is, based on the description of the PCR primer mix as a "PCR Primer Cocktail" and the fact that when I FastQC my sequencing data, I find sequences enriched in random hexamers in the first 9 positions of many reads. The reason I am in doubt is due to the fact, in my naive understanding, I can't see the need for the random hexamers since the transposons could carry specific sequences matching a given primer set(s).
Still, it would be great if someone could confirm or deny this.
Still, it would be great if someone could confirm or deny this.
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