Has anyone tried running beyond the 2x250bp "official" capacity of the MiSeq? We worked with a sequencing collaborator who hacked together a 2x250 run before the reagents were available by unofficially (although aided by Illumina tech support) pooling a 2x150 and a 1x100 cartridge. Any idea if pooling of a 2x250 and a 2x150 is possible, allowing early access to the supposedly upcoming 2x400 run?
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2 x 250 runs are not stably working (in our hands) as yet. Perhaps others have had better luck. No problems getting sequence, it is the decline in quality over the later cycles.
Hopefully this is just teething troubles that will get sorted out over time.
If you have the time (and money) to try the 2 x 400, go right ahead. Let us know what happens.
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Originally posted by GenoMax View Post2 x 250 runs are not stably working (in our hands) as yet. Perhaps others have had better luck. No problems getting sequence, it is the decline in quality over the later cycles.
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Phillip
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Originally posted by pmiguel View PostIs this with a hardware-upgraded instrument?
Does going to lower loading densities appear to help?
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Phillip
We are working with illumina at the moment. This may be a specific issue with our instrument. We shall see.
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