I am mapping out my journey into the RNA-seq world and would appreciate additional perspectives on the decision to use NuGEN or Truseq technologies for multiplexed RNA-seq on the Illumina HiSeq platform. I will be characterizing the transcriptome of a small subpopulation of cells that will be a challenge to acquire the 100ng of total RNA material that is suggested as a minimum for Truseq sample preparation.
Does Truseq prepared 100ng total RNA provide a much richer representation of the transcriptome than 10-25ng total RNA, that is more within reach, prepared with NuGEN Ovation system?
Does Truseq prepared 100ng total RNA provide a much richer representation of the transcriptome than 10-25ng total RNA, that is more within reach, prepared with NuGEN Ovation system?
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