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Hi all,
we are trying to do AmpliSeq on WGA from single cell. We tried a few times and to tell the story short, since our WGA fragments are between 150-200 bp we get lots of background noise that comes form the WGA fragment sthat are libraried together with the enriched targets. Do you have any suggestions to how to cope with these? would you increase the cycling? reduce the amount of starting material (currently using 30 ng)? Is there any way to modify the primers to then purify the captured products?
Thank you!
we are trying to do AmpliSeq on WGA from single cell. We tried a few times and to tell the story short, since our WGA fragments are between 150-200 bp we get lots of background noise that comes form the WGA fragment sthat are libraried together with the enriched targets. Do you have any suggestions to how to cope with these? would you increase the cycling? reduce the amount of starting material (currently using 30 ng)? Is there any way to modify the primers to then purify the captured products?
Thank you!
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