Dear all,
I need to perform ChIPSeq on a subpopulation of my neuroblastoma cell line. However, I am worried that the FACS will compromise the integrity of DNA protein interactions. I am going to look at chromatin marks as well as transcription factor myc.
Does anyone have experience with this topic? What nozzle size and pressure do you use for cell sorting? In our facility, we have an Aria I machine, and I would like to use a 85M nozzle.
Did anyone try and do the crosslinking before vs after the cell sorting?
Many thanks for any comments.
Mona
I need to perform ChIPSeq on a subpopulation of my neuroblastoma cell line. However, I am worried that the FACS will compromise the integrity of DNA protein interactions. I am going to look at chromatin marks as well as transcription factor myc.
Does anyone have experience with this topic? What nozzle size and pressure do you use for cell sorting? In our facility, we have an Aria I machine, and I would like to use a 85M nozzle.
Did anyone try and do the crosslinking before vs after the cell sorting?
Many thanks for any comments.
Mona
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