Hey guys, I'm a newbie to bioinformatics and still doing an undergrad. But I really needed help with something and hope you guys can. I am designing an expression vector using APE and needed to know about how to engineer the ribosome binding site into it. I'm sorry if this is a really dumb question but like I said, complete newbie.
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For this exercise you may want to resort to a low tech (pen and paper/talking this through with someone in person) method. Especially if you are new to informatics and do not have adequate biology background. Only you know what kind resources (vectors/fragments/enzymes) are available in the lab you are working in and unless you provide a lot of additional information it may not be possible for folks here to help.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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