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Old 01-12-2012, 05:05 PM   #1
sebbol
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Location: California

Join Date: Jan 2012
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Default HiC - crosslinking - glycine

hello. i have 2 questions.

1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)?

2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance?

thanks for reading the post and your kind help!

Seb
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