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Old 11-05-2012, 07:04 AM   #1
Bioo Scientific
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Default NEXTflex™ Pre-Capture Combo Kit Simplifies Sequence Target Capture Library Prep

The NEXTflex™ Pre-Capture Combo Kit is the first complete kit designed to work in combination with the Roche Nimblegen v3 SeqCap EZ Exome and EZ Choice Library kits. Bioo Scientific’s advanced library preparation technology, barcoded adapters and barcode blockers, all included in a single kit, make upstream processing of samples for targeted capture and enrichment easy and cost effective.

By using a library kit optimized for subsequent target enrichment, the user can expect comprehensive coverage of coding regions, more library, higher target enrichment efficiency and maximized variant discovery. Bioo Scientific is the only company that provides ready-to-use DNA Barcode Blockers for target capture. By providing the option of pre-capture or post-capture pooling of samples, the user is able to multiplex their samples efficiently, reducing sequencing costs and making high-throughput sequencing runs possible.

The kit incorporates “Enhanced Adapter Ligation Technology”, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientific’s robust series of NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior target enrichment and performance using the Nimblegen SeqCap EZ Library kits.
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Old 04-01-2013, 08:13 AM   #2
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Now incorporating barcode blockers for the NEXTflex-96™ DNA Barcodes, the NEXTflex™ Pre-Capture Combo Kit includes an option for multiplexing up to 96 samples at a time with NimbleGen’s SeqCap EZ Library target enrichment system. Kits are also available containing 6, 12 and 24 barcodes and barcode blockers for researchers with lower multiplexing requirements.
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Old 04-24-2013, 12:27 PM   #3
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Bioo Scientific recently released a whitepaper that demonstrates the efficacy of the NEXTflex™ Pre-Capture Combo Kit when used with the NimbleGen SeqCap EZ Library system for the construction of high quality libraries for Illumina-compatible sequencing. The whitepaper can be downloaded here.
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