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Old 05-26-2017, 06:30 AM   #1
Location: U.S.

Join Date: May 2017
Posts: 21
Default bluepippin size selection for SMRTbells


at which step of the 10kb protocol for preparation of SMRTbell libraries do you run BluePippin 20kb cassette for size selection? I am preparing gDNAs for complete sequencing.
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Old 05-26-2017, 03:44 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,236

Current 10kb protocol does not include a Pippin size selection step but if you want to include it then it will be after ligation clean ups. You might follow a Sequel 10 and 20kb hybrid protocol.
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Old 06-18-2017, 11:33 PM   #3
Location: Hong Kong

Join Date: Oct 2011
Posts: 46

With PacBio 10kb protocol, we got the library sizing around 7kb on agarose gel, no need to do size selection.
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library prep, pacbio, size selection, smrtbells

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