Tweet
Has anyone had a problem with the RFID reader with the NextSeq 500 sequencer? I believe this is the problem I am having with mine as it doesn't recognize or cannot read the flow cells when either trying to wash or run samples.
-
Todd D Prickett
NCI/NIH
Research Fellow
10 Center Dr
Building 10 CRC, rm. 3W-3864
Bethesda, MD 20892 -
We have run into this problem twice before. Illumina recommended power cycling the instrument (close out of all programs, hold power button on the front of instrument until it completely turns off, wait 5 minutes, power back on.). This has worked on both occasions. Good luck! -
This sounds like a good idea, thank you for the suggestion. I will post later if it works!Todd D Prickett
NCI/NIH
Research Fellow
10 Center Dr
Building 10 CRC, rm. 3W-3864
Bethesda, MD 20892Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment