I have another question about the bridge amplification / optimal insert size which is similar to the one asked by BTS about a week ago.
I know that the Illumina recommended insert size is around 150-300 bp (for single-read). And in the previous thread people mentioned that longer inserts worked well too. My question is that how short the insert can be? I only plan to use an isert with a size of about 60bp. Is this doable? Anybody has any experience?
Any input will be appreciated.
I know that the Illumina recommended insert size is around 150-300 bp (for single-read). And in the previous thread people mentioned that longer inserts worked well too. My question is that how short the insert can be? I only plan to use an isert with a size of about 60bp. Is this doable? Anybody has any experience?
Any input will be appreciated.
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