Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Illumina Hi/MiSeq turn around times

    We have 1 HiSeq and I was wondering for times when we are bottlenecked, which core facilities have capacity to serve other universities that can do human WGS on HiSeq or human exome capture. What are the typical turnaround times. Would it make a difference if I sent DNA or I did my own library prep? Rough cost of WGS versus exome (ballpark?). Turn around time is most important though.

    We sent off some WGS samples to the IGN which has an incredibly long turn around time (16-20 weeks). Often we do not care but in some cases it would be nice to have samples processed sooner.

  • #2
    This post might have been better suited for the service provider section as I suppose I am not opposed to a commercial entity doing the sequencing. I probably would prefer working with a core facility though.

    Comment


    • #3
      Perhaps we need to start a section where entities can post when they are likely to have spare capacity available and cost per run.

      I assume this would work best for ready to run libraries that can get on the instrument in a day or two after receipt.

      We generally have a backlog of libraries but sometimes find a machine or two idling for some time when libraries are not ready to run.

      Comment


      • #4
        Originally posted by GenoMax View Post
        Perhaps we need to start a section where entities can post when they are likely to have spare capacity available and cost per run.

        I assume this would work best for ready to run libraries that can get on the instrument in a day or two after receipt.

        We generally have a backlog of libraries but sometimes find a machine or two idling for some time when libraries are not ready to run.
        Here's a site that I have not used that can help facilitate selling extra capacity: http://blueseq.com/

        Comment


        • #5
          Hi Adaptive,

          Have you found spare capacity?

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Current Approaches to Protein Sequencing
            by seqadmin


            Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
            04-04-2024, 04:25 PM
          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 04-11-2024, 12:08 PM
          0 responses
          25 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 10:19 PM
          0 responses
          29 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-10-2024, 09:21 AM
          0 responses
          24 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 04-04-2024, 09:00 AM
          0 responses
          52 views
          0 likes
          Last Post seqadmin  
          Working...
          X