SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing



Similar Threads
Thread Thread Starter Forum Replies Last Post
ChIP-Seq: Enabling Data Analysis on High-Throughput Data in Large Data Depository Usi Newsbot! Literature Watch 1 04-18-2018 10:50 PM
demultiplexing fastq data(RNA-Seq data) abh Bioinformatics 0 11-07-2012 08:10 PM
Regression analysis of RNA-Seq data and qPCR data wilson90 Bioinformatics 2 08-30-2012 03:25 AM
Publicly available strand-specific RNA-Seq data with corresponding RT-PCR data? tmy1018 Bioinformatics 2 05-01-2012 01:50 PM
BCL to FASTQ conversation for paired-end RNA data seqmonkey Illumina/Solexa 8 09-27-2011 12:02 PM

Reply
 
Thread Tools
Old 11-07-2012, 08:27 PM   #1
abh
Member
 
Location: philadelphia

Join Date: Aug 2012
Posts: 13
Default demultiplexing fastq data(RNA-Seq data)

Hi,

I have 3 fastq files consisting of R1, R2 read files and
a separate index file. for example They are in the format below.

R1: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
Sequence data

R2: @M00132:6:000000000-A0JG4:1:1:18014:1842 2:N:0:0
Sequence data

Index: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
CTCGGT
+
<@@DFD

I want to split the R1 and R2 files based on this barcode.
and create new files that contain these barcodes

does any script or tool avaliable online so that i can work on it?

Last edited by abh; 11-08-2012 at 11:09 AM.
abh is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:56 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO