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Old 11-07-2012, 08:27 PM   #1
Location: philadelphia

Join Date: Aug 2012
Posts: 13
Default demultiplexing fastq data(RNA-Seq data)


I have 3 fastq files consisting of R1, R2 read files and
a separate index file. for example They are in the format below.

R1: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
Sequence data

R2: @M00132:6:000000000-A0JG4:1:1:18014:1842 2:N:0:0
Sequence data

Index: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0

I want to split the R1 and R2 files based on this barcode.
and create new files that contain these barcodes

does any script or tool avaliable online so that i can work on it?

Last edited by abh; 11-08-2012 at 11:09 AM.
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