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Thread | Thread Starter | Forum | Replies | Last Post |
miRNA mapping w/ Novoalign | quiteconfused | Bioinformatics | 1 | 05-22-2012 08:23 AM |
miRNA mapping w/ Novoalign | quiteconfused | RNA Sequencing | 0 | 05-21-2012 07:09 PM |
Can novoalign use FASTA as a reference format? (Specifically for mapping paired-end) | oiiio | Bioinformatics | 2 | 04-18-2011 10:47 AM |
Help:how to extrcate information from the output file of Novoalign mapping tools | qc.share | Illumina/Solexa | 2 | 09-20-2010 07:31 PM |
novoalign | xh4a | Bioinformatics | 6 | 06-15-2010 10:10 AM |
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#1 |
Member
Location: philadelphia Join Date: Aug 2012
Posts: 13
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Hi,
I want to use novoalign to map reads - allowing up to 15 mismatches for 100 bp paired-end reads I am new to novoalign(went through the manual) and confused with some of the options like -t,-x,-r etc.. so for 15 mismatches i should give something like -t 500 what would be -x then? this is the command line i am thinking to run novoalign -d genome -f A_1.fq A_2.fq -t 500 -r All -o SAM > out.sam Is this correct? thank you |
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