Hi everyone,
For an application I'm interested in, I would like to sequence libraries on Illumina (or Solid, I guess) starting from material containing both double and single-stranded DNA. If I'm not mistaken, standard library construction will miss single-stranded DNA, right?
Is anyone aware of a way to get single- and double- (or at least single- alone) stranded material represented in a library?
Thanks very much for your help!
For an application I'm interested in, I would like to sequence libraries on Illumina (or Solid, I guess) starting from material containing both double and single-stranded DNA. If I'm not mistaken, standard library construction will miss single-stranded DNA, right?
Is anyone aware of a way to get single- and double- (or at least single- alone) stranded material represented in a library?
Thanks very much for your help!
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