Hi,
I have 3 questions related with my fails in FastQC report.
1. I attached a printscreen presents graph with per base sequence content. Could tell me why I see deviation in the 3' end. Should I cut 3 bp on the 3' end?
2. My report shows red flag in Per tile sequence quality. I see few red tiles on the heatmap. What I should do with it?
3. I would like to use BWA-MEM to do alignment to reference genome. I heard that BWA-MEM expect min. length of read = 70 bp. Should I set MINLEN in Trimmomatic = 70?
I have 3 questions related with my fails in FastQC report.
1. I attached a printscreen presents graph with per base sequence content. Could tell me why I see deviation in the 3' end. Should I cut 3 bp on the 3' end?
2. My report shows red flag in Per tile sequence quality. I see few red tiles on the heatmap. What I should do with it?
3. I would like to use BWA-MEM to do alignment to reference genome. I heard that BWA-MEM expect min. length of read = 70 bp. Should I set MINLEN in Trimmomatic = 70?
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