Hi,
I used the cuffdiff to generating the differentiating analysis. However when I used the gtf file downloading from the ensembl, the cuffdiff gave me all non-significant results. All the p-values=1. Here is my code:
cuffdiff -o /Sequence/cuffdiff1_2 /hg19/Homo_sapiens.GRCh37.68.gtf /Sequence/cuffdiff1_2/sample1accepted.bam /Sequence/cuffdiff1_2/sample2accepted.bam
Any comments? Thanks!
I used the cuffdiff to generating the differentiating analysis. However when I used the gtf file downloading from the ensembl, the cuffdiff gave me all non-significant results. All the p-values=1. Here is my code:
cuffdiff -o /Sequence/cuffdiff1_2 /hg19/Homo_sapiens.GRCh37.68.gtf /Sequence/cuffdiff1_2/sample1accepted.bam /Sequence/cuffdiff1_2/sample2accepted.bam
Any comments? Thanks!