Greetings.
I have some material for a simple and very small, but useful, paper (described below). However, I have no idea where to publish it because all the journals I know are quite "big" - e.g. Bioinformatics, PLoS Computational Biology, Genome Research.
The paper is aimed at non-experts who work on my particular group of organisms or ones like it (medium-sized haploid and/or AT-rich genomes). The basis of the paper is that, using my results and shell scripted pipeline with a quick UNIX tutorial, any biologist can map and assess variants in a genome in way that is quick and light-weight and does not require the use of a web interface; they can do it like "real" bioinformaticians: fast, flexibly, and iteratively.
Basically, I have an assembled shell script that can be slightly modified for several different types of analyses, and outputs specially formatted results for what we would be looking for. The real trick is that it uses common tools (e.g. SAMtools, BFCtools) that are really for human (i.e. diploid and balanced AT) genomes and makes them effective tools for other organisms. I have tested all the different parameters and found the best ways to effectively find variants, which is different for these genomes from standard practices. There is nothing too revolutionary about this, but I definitely think it is a useful tool for people to set up their own pipelines and start to do the mapping in an intelligent way.
The work comes out of spending a year as a bioinformatics person and an experimental biologist at a medical research institution where people are really scared to work on a cluster or do anything command-line driven. I've taught the pipeline to plenty of people now and it seems to be working well.
Apologies if this is not the correct forum to search for guidance on this topic, I will happily switch if someone directs me to the correct place. Cheers and thanks.
I have some material for a simple and very small, but useful, paper (described below). However, I have no idea where to publish it because all the journals I know are quite "big" - e.g. Bioinformatics, PLoS Computational Biology, Genome Research.
The paper is aimed at non-experts who work on my particular group of organisms or ones like it (medium-sized haploid and/or AT-rich genomes). The basis of the paper is that, using my results and shell scripted pipeline with a quick UNIX tutorial, any biologist can map and assess variants in a genome in way that is quick and light-weight and does not require the use of a web interface; they can do it like "real" bioinformaticians: fast, flexibly, and iteratively.
Basically, I have an assembled shell script that can be slightly modified for several different types of analyses, and outputs specially formatted results for what we would be looking for. The real trick is that it uses common tools (e.g. SAMtools, BFCtools) that are really for human (i.e. diploid and balanced AT) genomes and makes them effective tools for other organisms. I have tested all the different parameters and found the best ways to effectively find variants, which is different for these genomes from standard practices. There is nothing too revolutionary about this, but I definitely think it is a useful tool for people to set up their own pipelines and start to do the mapping in an intelligent way.
The work comes out of spending a year as a bioinformatics person and an experimental biologist at a medical research institution where people are really scared to work on a cluster or do anything command-line driven. I've taught the pipeline to plenty of people now and it seems to be working well.
Apologies if this is not the correct forum to search for guidance on this topic, I will happily switch if someone directs me to the correct place. Cheers and thanks.
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