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Old 10-23-2014, 12:41 PM   #1
cheezemeister
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Default Amount of data for metagenomic analysis

Hello everyone

Just wondering if there is an amount of data that is generally considered acceptable for metagenomic analysis from 16S sequencing? Do I need >100,000 reads, or ? x number of Gb of data, for example?

Thanks!
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Old 11-23-2014, 05:52 PM   #2
kiss9499
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According to the Illumina protocol, >100,000 reads per sample is enough.
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Old 11-24-2014, 08:16 AM   #3
Brian Bushnell
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It really depends on the complexity of the community and how much of the low-abundance organisms you want to recover. There may not be a practical limit to how much you can sequence and still recover more, due to the exponential population distribution in many metagenomes.
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Old 11-24-2014, 01:31 PM   #4
nucacidhunter
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I would add link for this paper: http://www.nature.com/srep/2013/1306...srep01968.html
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