Hi, new to here, but hope someone may be able to offer some advice.
I'm currently thinking of designing an experiment whereby we sequence ~1000human samples through a 1.7Mb custom region using Agilent SureSelect.
2 strategies suggested are:
1) Pool DNA from 25 samples, make 1 library from the pooled DNA, analyse for variants using Syzygy, follow up called variants through the original 24 samples to identify which sample the variant originates in.
2) Barcode the samples from scratch which would require individual libraries for each +/- more lanes of sequencing?
Obviously option 2 makes follow up easier but significantly increases the costs and time required.
Does anyone have any experience of either method?
I'm currently thinking of designing an experiment whereby we sequence ~1000human samples through a 1.7Mb custom region using Agilent SureSelect.
2 strategies suggested are:
1) Pool DNA from 25 samples, make 1 library from the pooled DNA, analyse for variants using Syzygy, follow up called variants through the original 24 samples to identify which sample the variant originates in.
2) Barcode the samples from scratch which would require individual libraries for each +/- more lanes of sequencing?
Obviously option 2 makes follow up easier but significantly increases the costs and time required.
Does anyone have any experience of either method?
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