Metagenomics/Metatrancriptomics Sequencing

[newBioinfo]

Hello Everyone,
We have some ice samples and want to know where could we get it sequenced. We want to do metagenomics and metatranscriptomics.
Any kind of information will be highly appreciated.



Thanks!!!

[aarodriguezm]

Hi newBioinfo
There is a company in Germany calls GenXpro http://www.genxpro.info/
They have a very nice pool of services and good prices for what you may need.
Do not hesitate to contact them

[newBioinfo]

Thanks aarodriguezm,
I have contacted them and waiting for their reply.

[Francy87]

Hi newBioinfo!
What kind of analysis would you like to do? My university sequencing facility offers 16S amplicons sequencing and also bioinformatic analysis if you need!

[Francy87]

I forgot this: we use 454 technology, with amplicons of V1-V3 variable regions.
You can take a look to this:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0070222

[newBioinfo]

I forgot this: we use 454 technology, with amplicons of V1-V3 variable regions.
You can take a look to this:

http://www.plosone.org/article/info%...l.pone.0070222

Thanks Francy87,
We already have 16s rRNA data, now we want to do metagenomics and metatranscriptomics. Is you facility offering that.


Thanks!!

[Francy87]

Not yet, I'm sorry!

[genbio64]

NewBioinfo,
Our facility does offer that and we are stateside. I sent you a PM.

Hello Everyone,
We have some ice samples and want to know where could we get it sequenced. We want to do metagenomics and metatranscriptomics.
Any kind of information will be highly appreciated.



Thanks!!!

[rhinoceros]

Sorry, a little bit offtopic but what kind of sequencing are you planning? 454? Illumina? I've been wondering what would be optimal for metatranscriptomics and would it be the same for metagenomics as well. Perhaps MiSeq 2x300 with ~500 bp fragment size for metatranscriptomics at least? Of course all this would also depend on how much diversity you'd expect there is in your sample, etc.

[newBioinfo]

NewBioinfo,
Our facility does offer that and we are stateside. I sent you a PM.

Hi genbio64,
Could you provide us some details or contact info.

Thanks!!!

[lac302]

http://www.biotech.illinois.edu/htdna

i've used the university of illinois for 454 shotgun sequencing. good results and excellent customer service.

[newBioinfo]

http://www.biotech.illinois.edu/htdna

i've used the university of illinois for 454 shotgun sequencing. good results and excellent customer service.

Thanks lac302,
I checked the link and contacted them, could you tell me if they do the nucleic acid extraction also.

Thanks!!