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  • aligned and unaligned database

    Hi Everyone,
    I am very confused here, I am not able to get the difference between aligned and unaligned database.
    Here is an example of an aligned database:
    >AF515816.1
    .............................................................................................................................................................
    .............................................................................................................................................................
    .............................................................................................................................................................
    .............................................................................................................................................................
    .............................................................................................................................................................
    .............................................................................................................................................................
    .....................................................................................................A------T-G--A-C-G-C--T-G-G-C--G-G--C-A-T-G----------C---
    -T-T--TA-C--AC-A----T-G--C----A-A-G--T-C--GA-A-CG-----------G-CAG--C-G--G------------------------------------G-G-G-C-----------------------------------------
    -------------------------------------------------------------------------------------------------------------------------------------------------------------
    ---------------------------------------------TTC-G-----------------------------------------------------------------------------------------------------------
    -----------------------------------------------------------------------------------------------------------------------------------------------------------G-
    -C--C-T-------------------------G-C-C----G--GC--G-------AG-T-G-G-C-GA-A--C-------------G-G-------------------------------------------------------------------
    ------------------------------------------------------------------------------------------------------------------------------------------------G--T--G-A--G-
    T-A---AT-G-C-A--T-C-GG---A-A--C-GT-G--C-C-CAG--TC-A----------------------------------------------------------------------------------------------------------
    -------------------------------------------------------------------------------------------------------------------------------------------------------------
    -------------------------------------------------------------------------------------------------------------------------------------------------------------
    ------T-G--G----G--G--G---AT-AA-C--TA-------------------------C-G-C------------------------------------------------------------------------------------------
    -------------------------------------------------------------------------------------------------------------------------------------------------------------
    -------------------------------------------------------------------------------------------------------------------------------------------------------------
    -----------------------------------------------------------------------------------------------------------------------G--A-A-A-----------------------GCG-T-A
    -G-CT--A-A-TA---C-C-G--C-A--T-A----------C-------------------------------------------------------------------------------------------------------------------

    And the unaligned database looks like this:
    >S000406428 uncultured bacterium; BND5 Lineage=Root;rootrank;Bacteria;domain;"Actinobacteria";phylum;Actinobacteria;class;Acidimicrobidae;subclass;Acidimicr
    obiales;order;"Acidimicrobineae";suborder;Acidimicrobiaceae;family;Acidimicrobium;genus
    tgccggggactcgtaggggactgccggagttaattcggaggaaggtggggacgacgtcaagtcatcatgccccttacgtc
    cagggctgcacgcatgctacaatggccggtncaaagggtcgccaacccgcgagggggagccaatcccaaaaaaccggtct
    cagttcggatcggcagtctgcaactcgactgcttgaagtcggaktcgctagtaatcccggatcancacgccggggtgaat
    acgttcccgggccttgtacacaccgcccgt


    Please can anyone explain me the difference.
    Thanks for the help!!!!!!!!!

  • #3
    Hard to say without giving us more info. The unaligned version looks like it was downloaded from RDP. The other one doesn't, so I presume it was produced with some program. Then the dots could mean anything from gaps to no consensus change and masked region etc etc.

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