TopHat: output is not recognized by UCSC

geriatrics1200 · 06-03-2009, 08:44 AM

Hi, we ran TopHat without a GFF3 file and the output does not appear to be recognizable wiggle and bed formats. Here is what we got (first lines) of the wiggle file:
track type=bedGraph name="TopHat - read coverage"
gi|149258597|ref|NT_039457.7|Mm8_39497_37 0 46627 0
gi|149258597|ref|NT_039457.7|Mm8_39497_37 46627 46667 1
gi|149258597|ref|NT_039457.7|Mm8_39497_37 46667 48587 0
gi|149258597|ref|NT_039457.7|Mm8_39497_37 48587 48627 1

And here is what we got from the bed file:
track name=junctions description="TopHat junctions"
gi|149258597|ref|NT_039457.7|Mm8_39497_37 1132498 1133665 JUNC00000001
13 + 1132498 1133665 255,0,0 2 31,21 0,1146
gi|149258597|ref|NT_039457.7|Mm8_39497_37 1264693 1270688 JUNC00000002
39 + 1264693 1270688 255,0,0 2 32,32 0,5963

The program ran without errors. We think that there is a step missing that would associate the gi/NTs to chromosome coordinates. We are thus wondering what will be the way to work through this.. Is it because of the missing GFF3 file? (we could not find one for mouse, and if this is a problem, we would appreciate some pointers on how to make one) Any other suggestions?

Thanks very much in advance!

statsteam · 09-17-2009, 09:27 AM

I had the same problem before. What you need to do is to make your own bowtie index file using 'bowtie-build' command on fa files you downloaded from UCSC site.

Hope it helps,
Statsteam

bekkari · 11-06-2009, 11:13 AM

UCSC recognizes nothing but chr#, so have to you build your own indexes on chromosome sequences, which can downloaded from UCSC.

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06-03-2009, 08:44 AM	#1
geriatrics1200 Junior Member Location: boston, ma Join Date: Jun 2009 Posts: 3	TopHat: output is not recognized by UCSC Hi, we ran TopHat without a GFF3 file and the output does not appear to be recognizable wiggle and bed formats. Here is what we got (first lines) of the wiggle file: track type=bedGraph name="TopHat - read coverage" gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 0 46627 0 gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 46627 46667 1 gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 46667 48587 0 gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 48587 48627 1 And here is what we got from the bed file: track name=junctions description="TopHat junctions" gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 1132498 1133665 JUNC00000001 13 + 1132498 1133665 255,0,0 2 31,21 0,1146 gi\|149258597\|ref\|NT_039457.7\|Mm8_39497_37 1264693 1270688 JUNC00000002 39 + 1264693 1270688 255,0,0 2 32,32 0,5963 The program ran without errors. We think that there is a step missing that would associate the gi/NTs to chromosome coordinates. We are thus wondering what will be the way to work through this.. Is it because of the missing GFF3 file? (we could not find one for mouse, and if this is a problem, we would appreciate some pointers on how to make one) Any other suggestions? Thanks very much in advance! Last edited by geriatrics1200; 06-03-2009 at 09:43 AM.

11-06-2009, 11:13 AM	#3
bekkari Member Location: New Jersey Join Date: Oct 2009 Posts: 10	Yep, the same I heard from author UCSC recognizes nothing but chr#, so have to you build your own indexes on chromosome sequences, which can downloaded from UCSC.

09-17-2009, 09:27 AM	#2
statsteam Member Location: Californica Join Date: Sep 2009 Posts: 19	I had the same problem before. What you need to do is to make your own bowtie index file using 'bowtie-build' command on fa files you downloaded from UCSC site. Hope it helps, Statsteam