Hello All,
I am trying to figure out if ALLPATHS requires a 3kbp short jump insert size or if something larger like a 5 or 6kbp could be used in lieu of the 3kbp library.
The manual implies 3-10kbp will work but then I have read conflicting papers that indicate 3kbp is required but additional, larger, libraries can be added to increase power.
Maybe there is a drop off in assembly quality if you don't include a 3kbp library?
Thanks.
I am trying to figure out if ALLPATHS requires a 3kbp short jump insert size or if something larger like a 5 or 6kbp could be used in lieu of the 3kbp library.
The manual implies 3-10kbp will work but then I have read conflicting papers that indicate 3kbp is required but additional, larger, libraries can be added to increase power.
Maybe there is a drop off in assembly quality if you don't include a 3kbp library?
Thanks.
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