Hi guys!
I am visualizing bigwig files on the genome viewer (I use IGV) from RNAseq samples of human origin.
I have two clusters of 10 replicates each. I wanted to have just the experimental vs control track (otherwise it'll be too big screenshot, and also too confusing), but I am not sure what would be the good way to produce accurate data.
Should I use samtools merge to combine all the replicates from group#1 and group#2 (: all experimental combined and all control combined), and use those .bam files as input to create bigwig files? Or is there any other way?
Thanks to all!!!
Manu
I am visualizing bigwig files on the genome viewer (I use IGV) from RNAseq samples of human origin.
I have two clusters of 10 replicates each. I wanted to have just the experimental vs control track (otherwise it'll be too big screenshot, and also too confusing), but I am not sure what would be the good way to produce accurate data.
Should I use samtools merge to combine all the replicates from group#1 and group#2 (: all experimental combined and all control combined), and use those .bam files as input to create bigwig files? Or is there any other way?
Thanks to all!!!
Manu
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