Hi,
Illumina recently changed the data flow inside their HiSeq and NextSeq machines to reduce I/O burden during sequencing dramatically. It now skips saving .TIFF image files and .CIF intensity files by passing them through internal spaces in shared memory. Illumina technical support confirmed that there will be no way to get .CIF file or .TIFF images in HiSeq/NextSeq, soon.
As HiSeq Rapid V1, which is the last kit version that allows .CIF generation, is to be discontinued soon, there remains no commercially available kit in large-scale Illumina sequencing kits. MiSeq and GA IIx are still able to produce those files, but they don't meet the modern needs of throughput in many types of experiments.
If you're using .CIF files or images in your study, how do you deal with this changes? Is there anybody succeed accessing the shared memory or the "web HTTP interface" mentioned in the user guide?
Illumina recently changed the data flow inside their HiSeq and NextSeq machines to reduce I/O burden during sequencing dramatically. It now skips saving .TIFF image files and .CIF intensity files by passing them through internal spaces in shared memory. Illumina technical support confirmed that there will be no way to get .CIF file or .TIFF images in HiSeq/NextSeq, soon.
As HiSeq Rapid V1, which is the last kit version that allows .CIF generation, is to be discontinued soon, there remains no commercially available kit in large-scale Illumina sequencing kits. MiSeq and GA IIx are still able to produce those files, but they don't meet the modern needs of throughput in many types of experiments.
If you're using .CIF files or images in your study, how do you deal with this changes? Is there anybody succeed accessing the shared memory or the "web HTTP interface" mentioned in the user guide?
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