Syndicated from PubMed RSS Feeds
Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
PLoS One. 2011;6(5):e19723
Authors: Huang J, Zheng Z, Andersson AF, Engstrand L, Ye W
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
PMID: 21625543 [PubMed - in process]
More...
Rapid screening of complex DNA samples by single-molecule amplification and sequencing.
PLoS One. 2011;6(5):e19723
Authors: Huang J, Zheng Z, Andersson AF, Engstrand L, Ye W
Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample.
PMID: 21625543 [PubMed - in process]
More...