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The Micromon Recombinant DNA Techniques Course is running again this year, as it has for over 25 years. Have you been out of the lab for a while? Have a scientific background, but not that familiar with molecular biology? Want to learn some of the basic techniques and theory? Join us for this week-long intensive training course.
Hands-On Lab-Based Excercises:
The following applications are covered in the practical laboratory work spanning 30 hours of the workshop.
Lecture Series:
The following broad topics are covered in a series of 10 lectures throughout the week
Sunday 30 November – Friday 5 December, 2014
Venue: Microbiology Department, Monash University (Clayton), Australia.
Fee: AU$1900 (20% off for postgrad students) - lunch, morning tea and afternoon tea are included. 20% discount for postgraduate students
Visit our website for additional information and registration
.
Hands-On Lab-Based Excercises:
The following applications are covered in the practical laboratory work spanning 30 hours of the workshop.
- Cleavage and Ligation of Plasmid DNA
- Transformation of Bacterial Cells
- Preparation and Purification of Plasmid DNA
- Restriction Enzyme Mapping
- Agarose Gel Electrophoresis
- Recovery of DNA from Agarose Gels
- Preparation of Probes
- DIG-based Southern Blotting and Hybridisation
- Induction of Fusion Protein Production
- Polyacrylamide Gel Electrophoresis and Western Blotting
- Nucleic Acid Extraction
- Reverse Transcription and PCR
- Quantification of DNA and RNA by Bioanalysis
- Real-time PCR (qPCR)
- DNA Sequencing and Computer-aided Analysis
Lecture Series:
The following broad topics are covered in a series of 10 lectures throughout the week
- Introduction to Microbiology, DNA and Molecular Biology,
- Basic Cloning Requirements
- Gene Cloning Techniques
- Molecular Hybridisation (Northern, Southern and Western)
- Construction and screening of Genomic and cDNA Libraries
- Restriction Fragment Mapping
- Design and use of Oligonucleotide Primers
- Genetic Mapping
- DNA Sequencing
- Polymerase Chain Reaction and Real-Time PCR (qPCR)
- Site-directed Mutagenesis
- Cloning Vectors and their Applications
- Gene Expression Systems
- Basic Bioinformatics and Database Searching
Sunday 30 November – Friday 5 December, 2014
Venue: Microbiology Department, Monash University (Clayton), Australia.
Fee: AU$1900 (20% off for postgrad students) - lunch, morning tea and afternoon tea are included. 20% discount for postgraduate students
Visit our website for additional information and registration
.