Hi,
I sequenced by Illumina MiSeq some phage genomes. Normally, with bacterial genomes, I have an average sequencing coverage of 75-90x. But with the phages (much more small genomes) the coverages are more than 1500x.
I have read at some places that a too high coverage might be "problematic". Is someone can explain to me why?
Should I perform a random subsampling of my reads to assemble at a lowest average coverage (~100x)?
Thank you,
Antony
I sequenced by Illumina MiSeq some phage genomes. Normally, with bacterial genomes, I have an average sequencing coverage of 75-90x. But with the phages (much more small genomes) the coverages are more than 1500x.
I have read at some places that a too high coverage might be "problematic". Is someone can explain to me why?
Should I perform a random subsampling of my reads to assemble at a lowest average coverage (~100x)?
Thank you,
Antony
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