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Old 05-09-2017, 06:04 AM   #1
Location: Brasil

Join Date: Jun 2011
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Default Focus error in Miseq

We started a MiSeq running and after the first cycle there was an error: "Best focus is too near edge of range: AutoFocus would have moved z to 0, outside soft limits os -0,295001144426642... -0,00499885557335775: ThroughFocusSacnCommand".
Is it a camera problem?
May I start the running againg?

Thanks in advance for your attention!
frossit is offline   Reply With Quote
Old 05-09-2017, 06:45 AM   #2
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Contact Illumina tech support and let them take a look at your sequencer.
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Old 05-09-2017, 07:35 AM   #3
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There's no restarting a run with focus problems. It's unlikely to be a camera problem, more likely cluster failure, lens or z stage issue. Absolutely call tech support
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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Old 03-20-2018, 09:08 AM   #4
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We have had this error several times as well. The run just stops without images to show whether there is under- or over-clustering. I am very doubtful if this is just a clustering issue. I think it is a hardware problem.
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