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04-05-2018, 08:51 AM
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#1 |
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Senior Member
Location: East Coast USA Join Date: Feb 2008
Posts: 6,825
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Prevention of index hopping in Illumina tech
Illumina has announced free adapter blocking agents which are supposed to help minimize index hopping.
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04-05-2018, 11:43 AM
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#2 |
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Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,304
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Not clear what this is. Or what "minimizing index hopping" means. What fold reduction are they seeing with use of this reagent?
More info, including the manual here: https://support.illumina.com/sequenc...g-reagent.html As always, I'm less worried about "free adapter" per se. It's the adapter strands annealed to full length library molecules that tend to escape clean-up. -- Phillip |
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| illumina, index hopping |
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