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Old 02-23-2018, 10:11 AM   #1
jgibbons1
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Location: Worcester, MA

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Default nextera xt for 30 Mb genome

Hi all,

I was wondering if anyone has experience or has tested using the nextera xt kit for moderately sized eukaryotic genomes (20 - 40 Mb).

Any feedback would be greatly appreciated!

JG
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Old 02-23-2018, 03:29 PM   #2
nucacidhunter
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1 ng DNA of the organism will have 23,200-46,300 genome equivalents. Assuming 1Kb average fragment size and 25% conversion rate 1 ng DNA will result in 231M unique fragments. If you sequence the library below the unique fragment numbers to obtain the intended coverage then it should be fine.
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Old 02-24-2018, 11:50 AM   #3
jgibbons1
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I had not thought about it in that way. Thanks! This is very helpful.
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