Hi,
This should be a common thing to do, pooling a couple of genes in the same lane of solexa flowcell, as it still gives appropriate coverage.
The assumption being, that 30bp reads would map uniquely. How much of an issue is it? Say there are a few 40bp regions shared by the 2 genes. Is it fine to pool them in a lane (no tagging or indexing) or better use separate lanes for each gene?
This should be a common thing to do, pooling a couple of genes in the same lane of solexa flowcell, as it still gives appropriate coverage.
The assumption being, that 30bp reads would map uniquely. How much of an issue is it? Say there are a few 40bp regions shared by the 2 genes. Is it fine to pool them in a lane (no tagging or indexing) or better use separate lanes for each gene?
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