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I'd like to perform a targeted RNA-seq experiment to not only identify splice variants but also measure gene expression levels of the normal and abnormal variants I capture.
Does anyone have any suggestions or direct me to some publications that can provide some guidelines as to the number of housekeeping transcripts I should include in my capture design such that my bioinformatics group can provide me with expression levels of the targeted transcripts/novel splice variants I want to capture?
From my qPCR experience I was thinking that at least 3 would be needed but figured I'd ask the broader community to see if there was any consensus.
Does anyone have any suggestions or direct me to some publications that can provide some guidelines as to the number of housekeeping transcripts I should include in my capture design such that my bioinformatics group can provide me with expression levels of the targeted transcripts/novel splice variants I want to capture?
From my qPCR experience I was thinking that at least 3 would be needed but figured I'd ask the broader community to see if there was any consensus.