I asked Illumina about whether or not I could prepare my flowcell hyb cocktails well in advance and freeze them, and the official recommendation was not to store them for longer than a day or two. When I asked specifically why, they couldn't really tell me, but I got the impression that it might be related to loss of yield due to DNA sticking to the tubes, or perhaps instability of the sample in the hyb buffer. However, I could swear I've heard that labs do this regularly.
My question is: In the real world, do labs freeze (or otherwise store) samples in hyb buffer for later use? If so or if not, why? For how long?
Thanks!
My question is: In the real world, do labs freeze (or otherwise store) samples in hyb buffer for later use? If so or if not, why? For how long?
Thanks!
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