Hello,
Now I am trying to do RNA-Seq for DEG analysis between different tissues of plant root.
So far, I succeeded to extract total RNA (1-5 ng/ul in 10 ul, RIN ~ 7.0) from cryosectioned samples by using LCM technique.
I tried SMARTer kit and population Quartz-seq for cDNA synthesis and amplification, but both methods produced a strange peak which is longer than 10kb (High sensitivity DNA kit result attached). I have no idea what this peak is.
Has anyone seen this kind of huge peak? or Do you have any good ideas to make library using this sample?
Thanks
Now I am trying to do RNA-Seq for DEG analysis between different tissues of plant root.
So far, I succeeded to extract total RNA (1-5 ng/ul in 10 ul, RIN ~ 7.0) from cryosectioned samples by using LCM technique.
I tried SMARTer kit and population Quartz-seq for cDNA synthesis and amplification, but both methods produced a strange peak which is longer than 10kb (High sensitivity DNA kit result attached). I have no idea what this peak is.
Has anyone seen this kind of huge peak? or Do you have any good ideas to make library using this sample?
Thanks
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