hi,
i am working with a very limited amount of laser captured cells. RNA purification is extremely problematic in this setting, but direct lysis/cDNA amplification works very well.
how should i proceed from this first "classical" RT step to NGS?
thanks
pie
i am working with a very limited amount of laser captured cells. RNA purification is extremely problematic in this setting, but direct lysis/cDNA amplification works very well.
how should i proceed from this first "classical" RT step to NGS?
thanks
pie