Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Ray Error: miscount of the number of reads

    Dear all,

    I am trying to run Ray to de novo assemble a nematode genome.
    I run into the following error:

    Code:
    mpirun \
    -n 32 \
    /mnt/Programs/Ray-2.3.1/Ray \
    -k 81 \
    -o rayk81 \
    -p ../../clean_reads/PE_1.noCont.ec.fa ../../clean_reads/PE_2.noCont.ec.fa \
    -p ../../clean_reads/MP3_1.noCont.ec.fa ../../clean_reads/MP3_2.noCont.ec.fa \
    -p ../../clean_reads/MP5_1.noCont.ec.fa ../../clean_reads/MP5_2.noCont.ec.fa \
    -p ../../clean_reads/MP8_1.noCont.ec.fa ../../clean_reads/MP8_2.noCont.ec.fa
    
    [.....]
    
    Rank 7: File ../../clean_reads/MP8_2.noCont.ec.fa (Number 7) has 10233322 sequences
    Rank 6: File ../../clean_reads/MP8_1.noCont.ec.fa (Number 6) has 10231913 sequences
    Rank 5: File ../../clean_reads/MP5_2.noCont.ec.fa (Number 5) has 10722610 sequences
    Rank 2: File ../../clean_reads/MP3_1.noCont.ec.fa (Number 2) has 14151655 sequences
    Rank 4: File ../../clean_reads/MP5_1.noCont.ec.fa (Number 4) has 10722031 sequences
    Rank 3: File ../../clean_reads/MP3_2.noCont.ec.fa (Number 3) has 14152522 sequences
    Rank 0: File ../../clean_reads/PE_1.noCont.ec.fa (Number 0) has 100860164 sequences
    Rank 1: File ../../clean_reads/PE_2.noCont.ec.fa (Number 1) has 100860164 sequences
    Rank 0 wrote rayk81/NumberOfSequences.txt
    Rank 0 wrote rayk81/SequencePartition.txt
    
    Rank 0 : Error, ../../clean_reads/MP3_1.noCont.ec.fa contains 14151655 sequences and ../../clean_reads/MP3_2.noCont.ec.fa contains 14152522 sequences (must be the same)
    The problem detected by Ray (not the same number of sequences in the left and right read files) is wrong. Actually Ray does not seem to correctly count the sequences:

    Code:
    grep -c '^>' ../../clean_reads/MP3_1.noCont.ec.fa
    9763950
    grep -c '^>' ../../clean_reads/MP3_2.noCont.ec.fa  
    9763950
    A head of my read files looks completely normal (regular multiline fasta).
    I have also run other assemblers successfully on this data, so I know there is no format problem with the files.

    Any insights on what could be causing this problem?

    Best Wishes,
    Sophie

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-10-2024, 06:35 AM
0 responses
20 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-09-2024, 02:46 PM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-06-2024, 07:17 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Working...
X