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Old 11-27-2008, 06:00 AM   #1
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Default PubMed: A large genome center's improvements to the Illumina sequencing system.

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Related Articles A large genome center's improvements to the Illumina sequencing system.

Nat Methods. 2008 Dec;5(12):1005-10

Authors: Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ, Durbin R, Swerdlow H, Turner DJ

The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.

PMID: 19034268 [PubMed - in process]



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