Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • P.F.A. versus Formaldehyde

    Hello all. I am trying to perform ChIP seq for the first time and was wondering whether using paraformaldehyde (PFA) versus regular formaldehyde makes a huge difference wit respect to fixation efficiency ? I currently use P.F.A (37%) and crosslink for 8 minutes, after which, I quench with glycine for 5 minutes. When opening the P.F.A. bottle for the first time, I transfer the liquid to a 15ml conical tube, and use it on my cells. After usage I store at -20 Celsius, until further use. After the second time I use it, I dilute it down to 4% and store at 4 degree. Would using 37% P.F.A in this manner decrease crosslinking efficiency?

    -M.N.

  • #2
    Paraformaldehyde is the solid form, when its dissolved in aqueous solution it becomes formaldehyde. Formalin is 37% formaldehyde that is usually sold in solution and from what I read also contains a little methanol.

    Many histologists tell you you should use para and make it fresh from the solid to preserve antigens, but not sure how good the evidence is for this.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    10 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    9 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    51 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    67 views
    0 likes
    Last Post seqadmin  
    Working...
    X