subtraction of human genome

[doggy]

Hello,

I am using CLC bio for a metagenomic analysis.

However, the largest part of the reads is from the human genome.

Therefore, I have to exclude the reads which align to the human genome,
at the first hand (so called digital subtraction).

CLC suggested me to use the following feature:

http://www.clcsupport.com/clcgenomicsworkbench/current/index.php?manual=Mapping_output_options.html

However, it turned out that, running this feature would require 24 - 48 GB
of computer memory.

I cannot afford such a high-spec machine.

Is it possible to run this feature on a machine with only 8GB memory,
or are there any websites which could kindly carry out this digital subtraction?

Thanks

[dpryan]

You're paying CLC for support, you should ask them. The community will generally not provide support for commercial software packages.